Introducing the Cas9 ELISA Assay
Clustered regularly interspaced short palindromic repeats, or CRISPR, is a term used to describe repeating sequences of DNA in prokaryotc organisms. Cas9 is a protein that takes advantage of these repeating sequences to edit the genome in organisms. The applications of the CRISPR-Cas9 system are endless in the Life Sciences field.
Researchers have the ability to target specific genes and turn them on or off, or even modify the gene. There are vast implications for genetic desease research, development of optimal lab animal models, and even environmental science research with the use of the CRISPR/Cas9 system.
- XpressBio has developed an assay that can detect as little as 1.1ng/ml of S. pyogenes Cas9 protein in experimental samples including cell culture and tissue (plant and animal) lysates.
- Additionally, our Cas9 ELISA is ideal for evaluating transfection efficiency for CRISPR/Cas9 system incorporation into the targeted cell or tissue populations.
- Our easy-to-follow protocol and removable ELISA strips make this kit a valuable tool for your genome-editing research.
Method of Use
Test specimen added to coated microtitration wells. Cas9 antigen in specimen binds to murine anti-Cas9 capture antibody coated wells.
After incubation and wash, biotinylated mouse monoclonal Cas9 detection antibody is added to wells. Detection antibody binds with capture antibodyspecimen antigen complex.
After 2nd incubation and wash, Streptavidin HRP enzyme conjugate is added to wells. Enzyme conjugate binds specifically to detector antibody.
After 3rd incubation and wash, immediately add TMB substrate solution. Substrate solution will react with specifically bound enzyme conjugate.
After 4th incubation, add stop solution. The solution in the wells will change from blue to yellow. Use microtitration plate reader set at 450nm to determine levels of Cas9 reactive determinants in specimens.
Murine anti-Cas9 capture antibody
Cas9 antigen in specimen
Biotinylated mouse monoclonal Cas9 detector antibody
Streptavidin HRP enzyme conjugate
TMB substrate solution