Each plate is coated with a calicivirus based on its characteristic morphology, its positive stranded RNA genome and especially by the presence of a single structural polypeptide of 60kD (VP60). It is a recombinant VP60 protein expressed in baculovirus which is used to coat microtiter plates.
Rabbit Haemorrhagic Disease Virus (RHDV), also known as rabbit calicivirus, is an ssRNA virus of the family Calciviridae, genus Lagovirus, that causes Rabbit Haemorrhagic Disease in adult rabbits. The virus is highly contagious and extremely lethal in rabbits and has a worldwide distribution. Transmission can occur via direct contact or fomites, and the virus most commonly enters the host through the oral route. Virus can also be transported via insect, birds or mammals other than rabbits. Rabbit Haemorrhagic disease may result in death without any observable symptoms and mortality rates are high depending on the strain of the virus. Pathological changes may present in almost any organ system and cause multiple organ failure. Laboratory research can be severally affected by infection with RHDV.
Abrantes J, van der Loo W, Le Pendu J, Esteves PJ (2012) Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review. Vet Res 43: 12.
Baker DG. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clin Microbiol Rev. 1998;11:231–266.
Abrantes J, van der Loo W, Le Pendu J, Esteves PJ. Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review. Vet Res. 2012;43:12.