Description
Express Capture Protein A Coated Microplates provide a solid-phase surface for capturing immunoglobulins through affinity interactions between immobilized Protein A and the Fc region of compatible antibodies. Fc-directed capture helps position the antigen-binding regions away from the plate surface so they remain available to bind target molecules in solution.
Protein A binds immunoglobulins in a species-, class-, and subclass-dependent manner. It is commonly used with rabbit IgG and may also provide useful binding with antibodies from several other mammalian species. Protein A generally binds mouse IgG1 and most rat IgG subclasses weakly; compatibility should therefore be confirmed for the antibody used in the assay.
The plates are intended for research immunoassays in which an immobilized antibody captures a labeled or otherwise detectable antigen. Detection may be performed with enzyme-labeled, biotinylated, fluorescent, chemiluminescent, or comparable reagents.
